Filter DNA tool: Removes non-DNA characters, such as numbers and blank spaces, from the text of a DNA sequence, making it ready to copy and paste into other applications.DNA sequence reverse complement tool: Enter a DNA sequence and the program will give you the reverse sequence, complement sequence, or reverse and complement sequence.Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze them. Codon optimization tool: To access this tool, set up an IDT account.įigure 2: Addgene's Analyze Sequence tool.UniProt: A database of protein sequence and functional information, such as expression patterns, post-translational modifications and polymorphisms.ExPASy translation tool: Translates a DNA sequence using all six open reading frames.NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database.Addgene vector database: Find sequences for plasmids assembled from publications and commercially available sources, as well as those deposited with Addgene.Webcutter 2.0: For finding restriction enzyme cut sites.NEBCutter2: For finding restriction enzyme cut sites.Addgene's analyze sequence tool: Annotates features, BLASTs, aligns, digests, and translates a sequence.Wayne Davis from the University of Utah.įigure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA.ĭNA / protein database search and analysis tools ApE (A plasmid Editor): A free, donation-based plasmid analysis tool including editing, annotating, creating maps, and more.
#SNAPGENE VIEWER. SERIAL#
Serial Cloner: Free desktop-based software for plasmid design and mapping.Benchling: While you might think of Benchling as an electronic lab notebook, it also has a suite of molecular biology tools and can make plasmid maps.SnapGene Viewer: The free SnapGene Viewer is great for looking at plasmid maps and viewing sequencing traces, while the paid version provides more tools for plasmid mapping and design (Figure 1).These tools are for viewing, editing or making plasmid maps, but can also analyze and annotate any DNA sequence. To help you keep your virtual molecular biology toolbox organized, today’s post features a list of free online molecular biology tools all in one place. We all have our favorite tools for tackling these particular tasks, but they tend to be scattered about the internet.
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